Mouse acylation stimulating protein (ASP) ELISA kit

Cat. No.: OB0625WXX-196

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: ASP

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Mouse acylation stimulating protein (ASP) ELISA kit

INQUIRY Datasheet MSDS

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Product Overview

Description: Mouse acylation stimulating protein (ASP) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of ASP in mouse with a range of 0.156-10 ng/mL and a specificity of up to 0.05 ng/mL.

Assay Principle: The pre-coated antibody is a mouse ASP monoclonal antibody, and the detection antibody is a biotin-labeled polyclonal antibody. The samples and biotin-labeled antibodies are added to the ELISA plate wells and rinsed with PBS or TBS. Avidin-peroxidase conjugate is then sequentially added to the ELISA wells; the reactants are thoroughly rinsed with PBS or TBS and then colored with TMB substrate. TMB turns blue when catalyzed by peroxidase and finally turns yellow in the presence of acid. The shade of color is positively correlated with the detection factor in the sample.

Background: ASP has the effects of enhancing glucose transport and inhibiting hormone-sensitive lipase, and is associated with the pathogenesis of obesity.

Synonyms: Acyl stimulation protein

Applications: Mouse acylation stimulating protein (ASP) ELISA kit is used to quantify ASP in mouse serum, plasma, and cell culture supernatant samples of mouse, providing data to support a wide range of studies.

Research Area: Metabolic pathway; Obesity research

Specification

Sample Type: Mouse serum; Plasma; Cell culture supernatant

Detection Range: 0.156-10 ng/mL

Sensitivity: Up to 0.05 ng/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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