Human neurotensin receptor 2 (NTSR2) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-1185

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: NTSR2

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Human neurotensin receptor 2 (NTSR2) ELISA kit (0.156-10 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Human neurotensin receptor 2 (NTSR2) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of NTSR2 in human samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.061 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The provided microtiter plate is pre-immobilized with an antibody specific to NTSR2. Standards or samples are introduced into the designated microtiter wells along with a biotin-conjugated detection antibody preparation specific for NTSR2. Subsequently, avidin-conjugated HRP is added to each microplate well and incubated. After the TMB substrate solution is introduced, color development occurs exclusively in wells containing the NTSR2 complex. A sulphuric acid solution terminates the enzyme-substrate reaction, and the absorbance is recorded at 450 nm ± 10 nm. The final concentration of NTSR2 is calculated by mapping the OD values against the established standard curve.

Background: NTSR2 is a G protein-coupled receptor and one of the receptors for the neuropeptide neurotensin. It influences metabolic products and is associated with pathological processes such as obesity.

Synonyms: NTR2; Neurotensin receptor type 2; Levocabastine-sensitive neurotensin receptor; NT-R-2; NTR2

Applications: Human neurotensin receptor 2 (NTSR2) ELISA kit (0.156-10 ng/mL) is used to quantify NTSR2 in tissue homogenates and other biological fluids of human, providing data to support research in a wide range of areas, including energy balance, signal transduction, obesity, etc.

Research Area: Energy balance; Signal transduction; Obesity

Specification

Sample Type: Tissue homogenates; Other biological fluids

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.061 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (others).

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