Pig C-reactive protein (CRP) ELISA kit (0.156-10 ng/mL)

Cat. No.: OB0625WXX-454

Assay Type: Quantitative ELISA

Target Species: Pig

Assay Target: CRP

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Pig C-reactive protein (CRP) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Pig C-reactive protein (CRP) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CRP in pig with a range of 0.156-10 ng/mL and a specificity of <0.055 ng/mL.

Assay Principle: The microtiter plate provided in this kit is pre-coated with an antibody specific to the target antigen. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody preparation specific to the target antigen, and avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter plate well and incubated. TMB substrate solution is then added to each well. Only wells containing the target antigen, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 2 nm. The concentration of the target antigen in the sample can then be determined by comparing the OD of the sample to a standard curve.

Background: CRP is an acute-phase protein of hepatic origin, whose circulating concentrations rise in response to inflammation. It is considered a key plasma protein that can bind to Leptin, and its potential role in regulating leptin's function in obesity is crucial.

Synonyms: Pentraxin-related; pentraxin 1; C-RP; PTX1

Applications: Pig C-reactive protein (CRP) ELISA kit (0.156-10 ng/mL) is used to quantify CRP in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluid samples of pig, providing data to support a wide range of studies.

Research Area: Inflammatory reaction; Obesity research

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell culture supernates; Other biological fluids

Detection Range: 0.156-10 ng/mL

Sensitivity: <0.055 ng/mL

Precision (Intra-assay): CV≤4.6%

Precision (Inter-assay): CV≤8.2%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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