Monkey lipopolysaccharide binding protein (LBP) ELISA kit (2.5-50 ng/mL)

Cat. No.: 0126WXX-766

Assay Type: Quantitative sandwich ELISA

Target Species: Monkey

Assay Target: LBP

Size:

Monkey lipopolysaccharide binding protein (LBP) ELISA kit (2.5-50 ng/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Monkey lipopolysaccharide binding protein (LBP) ELISA kit (2.5-50 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LBP in monkey samples with a range of 2.5-50 ng/mL and a minimum detectable dose (sensitivity) of 1.0 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The method employs a monoclonal anti-LBP antibody and an LBP-HRP conjugate. Samples and assay buffers are co-incubated with the LBP-HRP conjugate in a pre-coated microplate for one hour. During this process, LBP within the sample competes with the conjugate for a limited number of antibody binding sites. After incubation, the wells are washed five times to remove unbound reagents. An HRP-specific substrate is then added to produce a blue complex, which shifts to yellow upon the addition of a stop solution. The resulting color intensity, measured at 450 nm, is inversely proportional to the LBP concentration. A standard curve is utilized to interpolate the quantitative value of LBP for each sample.

Background: LBP is a secreted protein primarily present in the extracellular space. Its core function is to bind bacterial lipopolysaccharide (LPS), a glycolipid found on the outer membrane of all Gram-negative bacteria. LBP is significant in obesity research because it plays a pivotal role in the link between inflammation, gut microbiota, and metabolic health. By studying LBP's mechanisms of action and its level changes, scientists can better understand how obesity leads to systemic inflammation and insulin resistance, potentially developing therapies targeting LBP or its associated signaling pathways.

Synonyms: LPS-binding protein

Applications: Monkey lipopolysaccharide binding protein (LBP) ELISA kit (2.5-50 ng/mL) is used to quantify LBP in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of monkey, providing data to support research in a wide range of areas, including Infection immunity, obesity, etc.

Research Area: Infection immunity; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 2.5-50 ng/mL

Sensitivity: 1.0 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out