Human tumor-associated antigen (TAA) ELISA kit-Quantitative sandwich

Cat. No.: 0126WXX-1863

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: TAA

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Human tumor-associated antigen (TAA) ELISA kit-Quantitative sandwich

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Product Overview

Description: Human tumor-associated antigen (TAA) ELISA kit-Quantitative sandwich is an ELISA-based in vitro research tool designed specifically for the quantitative detection of TAA in human samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of <0.05 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes the double-antibody Sandwich ELISA technique. The microplate is pre-coated with an anti-human TAA monoclonal antibody, while the detection agent is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added to the ELISA plate wells and subsequently washed with PBS or TBS to remove unbound material. Following this, avidin-peroxidase conjugates are introduced into the wells. After the enzyme conjugate has been thoroughly washed out with PBS or TBS, TMB substrate is added for coloration. The TMB reacts under peroxidase activity to form a blue product, which finally shifts to yellow upon the addition of the stop solution (color reagent C). The resulting color intensity is positively correlated with the quantity of the target analyte present in the sample.

Background: TAAs are a class of endogenous proteins that are highly or abnormally expressed in tumor cells but are present at extremely low levels or absent in normal cells. They form the basis for the immune system's recognition of cancer cells and serve as primary targets for tumor immunotherapy and diagnostic biomarkers. Concurrently, TAAs are key molecules in investigating how obesity influences antitumor immune responses.

Synonyms: Tumor antigen; TA

Applications: Human tumor-associated antigen (TAA) ELISA kit-Quantitative sandwich is used to quantify TAA in serum, plasma, and cell culture supernatant of humans, providing data to support research in a wide range of areas, including immunology, among others.

Research Area: Immunology