Human transient receptor potential Cation channel subfamily V member 1 (TRPV1) ELISA kit (0.312-20 ng/mL)

Cat. No.: 0126WXX-2009

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: TRPV1

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Human transient receptor potential Cation channel subfamily V member 1 (TRPV1) ELISA kit (0.312-20 ng/mL)

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Product Overview

Description: Human transient receptor potential Cation channel subfamily V member 1 (TRPV1) ELISA kit (0.312-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of TRPV1 in human samples with a range of 0.312-20 ng/mL and a minimum detectable dose (sensitivity) of <0.078 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a quantitative sandwich enzyme immunoassay format. An antibody specific for TRPV1 has been pre-immobilized onto a microplate. Standards and samples are pipetted into the wells, allowing any TRPV1 present to be captured by the stationary antibody. After removing unbound substances through a wash step, a biotin-conjugated detection antibody specific for TRPV1 is introduced. Following a subsequent wash, Avidin-conjugated HRP is added. After a final wash to remove any unbound reagents, a substrate solution is added, and color develops in direct proportion to the amount of TRPV1 captured in the initial step. The reaction is stopped, and the intensity of the color is recorded.

Background: Transient receptor potential cation channel subfamily V member 1 (TRPV1) is a non-selective cation channel and receptor for capsaicin, functioning as a transducer of thermal and chemical stimuli in sensory neurons. By mediating calcium influx and downstream signaling, TRPV1 influences energy metabolism, thermogenesis, and appetite regulation. Altered TRPV1 activity has been associated with changes in metabolic rate and energy balance, linking it to obesity and related metabolic disorders.

Synonyms: Vanilloid receptor subtype 1; Capsaicin receptor; Osm-9-like TRP channel 1; Vanilloid receptor 1; VR1; OTRPC1

Applications: Human transient receptor potential Cation channel subfamily V member 1 (TRPV1) ELISA kit (0.312-20 ng/mL) is used to quantify TRPV1 in serum, plasma, tissue homogenates, and cell lysates of human, providing data to support research in a wide range of areas, including signal transduction, obesity, and others.

Research Area: Signal transduction; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates.

Detection Range: 0.312-20 ng/mL

Sensitivity: <0.078 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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