Human prothrombin ELISA kit (0.5-10 ng/mL)

Cat. No.: 0126WXX-1588

Assay Type: Quantitative ELISA

Target Species: Human

Assay Target: Prothrombin

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Human prothrombin ELISA kit (0.5-10 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Human prothrombin ELISA kit (0.5-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of prothrombin in human samples with a range of 0.5-10 ng/mL and a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The prothrombin ELISA kit employs a quantitative sandwich enzyme immunoassay technique. The microtiter plate is pre-immobilized with a monoclonal antibody specific for prothrombin. Standards or samples are introduced into the wells, where any prothrombin present binds to the capture antibody. To quantify the target, a standardized preparation of HRP-conjugated polyclonal antibody specific for prothrombin is added to each well to "sandwich" the immobilized prothrombin. After incubation, the microtiter plate is thoroughly washed to remove all unbound components. Subsequently, substrate solutions are added, allowing the enzyme and substrate to react. Color development occurs exclusively in wells containing the prothrombin-antibody complex. The reaction is terminated with a sulphuric acid solution, and the absorbance is measured at 450 nm. A standard curve is plotted to relate the color intensity (O.D.) to the standard concentrations, from which the prothrombin concentration in each sample is interpolated.

Background: Prothrombin is a vitamin K-dependent glycoprotein synthesized by the liver and serves as a key precursor in the blood coagulation cascade. Upon activation, it converts to thrombin, a core serine protease essential for forming fibrin clots and activating other coagulation factors. This process is vital for maintaining blood homeostasis, inflammatory responses, and wound healing. Prothrombin is closely associated with obesity-related complications.

Synonyms: Coagulation factor II; F2

Applications: Human prothrombin ELISA kit (0.5-10 ng/mL) is used to quantify prothrombin in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of human, providing data to support research in a wide range of areas, including inflammation, vascular biology, obesity, and others.

Research Area: Inflammation; Vascular biology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 0.5-10 ng/mL

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 94-103%

Storage: Store at 2-8°C.

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