Human peroxisome proliferator-activated receptor gamma (PPARγ) ELISA kit (0.16-10 ng/mL)

Cat. No.: 0126WXX-1534

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: PPARγ

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Human peroxisome proliferator-activated receptor gamma (PPARγ) ELISA kit (0.16-10 ng/mL)

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Product Overview

Description: Human peroxisome proliferator-activated receptor gamma (PPARγ) ELISA kit (0.16-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PPARγ in human samples with a range of 0.16-10 ng/mL and a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This ELISA kit utilizes the sandwich-ELISA principle. The microplate provided is pre-coated with an antibody specific to Human PPARγ. Standards or samples are added to the wells to bind with the capture antibody. A biotinylated detection antibody and an avidin-HRP conjugate are added successively and incubated. After washing away free components, a substrate solution is introduced. Wells containing the target will appear blue and then turn yellow upon the addition of the stop solution. The optical density (O.D.) is measured at 450 ± 2 nm, and the value is directly proportional to the concentration of Human PPARγ.

Background: PPAR-gamma (Peroxisome Proliferator-Activated Receptor Gamma), a prominent member of the nuclear hormone receptor superfamily located at chromosomal position 3p25, functions as a master ligand-dependent transcription factor essential for adipogenesis, lipid homeostasis, and glucose metabolism. Preferentially expressed in adipocytes, vascular smooth muscle cells, and macrophages, this DNA-binding nuclear receptor operates as a heterodimer with the retinoid X receptor and interacts with coactivators such as NCOA6 to significantly enhance the transcription of target genes. Its biological activity, which is modulated by MAP kinase-mediated phosphorylation and insulin stimuli, governs critical processes including white and brown fat cell differentiation, long-chain fatty acid transport, and the regulation of systemic insulin sensitivity. Beyond its primary metabolic roles, PPAR-gamma serves as a receptor for fatty acids and hypolipidemic drugs, influencing diverse physiological pathways ranging from the negative regulation of inflammation and smooth muscle cell proliferation to the orchestration of circadian rhythms and blood pressure. Consequently, dysregulation or genetic variation in PPAR-gamma is fundamentally linked to the pathogenesis of obesity, non-insulin-dependent diabetes mellitus, and familial partial lipodystrophy, underscoring its pivotal role as a central hub in metabolic health and disease.

Synonyms: PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone receptor; Nuclear receptor subfamily 1 group C member 3; PPARg; Peroxisome proliferator activated receptor gamma; PPAR-γ; Peroxisome proliferator-activated receptor γ

Applications: Human peroxisome proliferator-activated receptor gamma (PPARγ) ELISA kit (0.16-10 ng/mL) is used to quantify PPARγ in serum, plasma, and other biological fluids of humans, providing data to support research in a wide range of areas, including metabolic pathways, endocrinology, cardiovascular biology, developmental science, bone metabolism, obesity, and others.

Research Area: Metabolic pathway; Endocrinology; Cardiovascular biology; Developmental science; Bone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 0.16-10 ng/mL

Sensitivity: 0.1 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 87-109%

Storage: Store at 2-8°C.

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