Human Perilipin 1 (PLIN1) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-1364

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: PLIN1

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Human Perilipin 1 (PLIN1) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Human Perilipin 1 (PLIN1) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PLIN1 in human samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.054 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microplate included in this kit is pre-coated with an antibody specific to PLIN1. Standards or samples are introduced into the designated wells along with a biotin-labeled detection antibody specific for PLIN1. Next, avidin-conjugated HRP is added and incubated. Upon the addition of TMB substrate, only the wells containing the PLIN1-biotin-avidin complex will demonstrate a colorimetric change. The enzymatic reaction is terminated with a sulphuric acid solution, and the absorbance is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of PLIN1 in the specimens is determined by mapping the O.D. results against the standard curve.

Background: PLIN1 is an adipocyte-specific lipid-binding protein that localizes to lipid droplets and plays a central role in regulating lipid storage and mobilization. By coating lipid droplets, PLIN1 protects stored triacylglycerols from basal hydrolysis while enabling controlled lipolysis in response to hormonal stimulation through phosphorylation by cAMP-dependent protein kinase. This dynamic regulation of lipid breakdown is essential for maintaining adipocyte lipid homeostasis. Dysregulation or mutation of PLIN1 impairs normal lipolytic control and fat storage, contributing to abnormal adipose tissue function and is closely associated with obesity and related metabolic disorders, including familial partial lipodystrophy.

Synonyms: PERI; Lipid droplet associated protein

Applications: Human Perilipin 1 (PLIN1) ELISA kit (0.156-10 ng/mL) is used to quantify PLIN1 in tissue homogenates, cell lysates, and other biological fluids of humans, providing data to support research in a wide range of areas, including metabolic pathways, obesity, and others.

Research Area: Metabolic pathway; Obesity

Specification

Sample Type: Tissue homogenates; Cell lysates; Other biological fluids

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.054 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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