Human neurotrophic tyrosine kinase receptor type 2 (NTRK2) ELISA kit (93.75-6000 pg/mL)

Cat. No.: 0126WXX-1181

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: NTRK2

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Human neurotrophic tyrosine kinase receptor type 2 (NTRK2) ELISA kit (93.75-6000 pg/mL)

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Product Overview

Description: Human neurotrophic tyrosine kinase receptor type 2 (NTRK2) ELISA kit (93.75-6000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of NTRK2 in human samples with a range of 93.75-6000 pg/mL and a minimum detectable dose (sensitivity) of 56.25 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is based on sandwich ELISA technology. An anti-human NTRK2 antibody is pre-coated onto 96-well plates, and a biotin-conjugated anti-human NTRK2 antibody is employed as the detection antibody. Standards, test samples, and the biotinylated detection antibody are added to the wells sequentially, followed by a wash step with wash buffer. HRP-streptavidin is then introduced, and unbound conjugates are removed through further washing. TMB substrates are used to visualize the HRP enzymatic reaction; the TMB is catalyzed by HRP to produce a blue product that shifts to yellow upon the addition of an acidic stop solution. The intensity of the yellow color is directly proportional to the amount of Human NTRK2 captured in the plate. The O.D. absorbance is measured at 450 nm in a microplate reader, allowing for the calculation of the Human NTRK2 concentration.

Background: NTRK2, also known as TrkB, is a high-affinity receptor for brain-derived neurotrophic factor (BDNF). The BDNF/TrkB signaling pathway is crucial for neuronal survival, differentiation, and plasticity in the central nervous system (CNS). The link between NTRK2 and obesity research primarily manifests in the CNS regulation of appetite: BDNF serves as a key anorexigenic (appetite-suppressing) signal. Defects in NTRK2 expression and function in the hypothalamus weaken BDNF signaling, leading to overeating, reduced energy expenditure, and severe obesity. Consequently, NTRK2 serves as a key signaling integration point in central obesity regulation pathways (acting synergistically with the MC pathway, leptin signaling, etc.).

Synonyms: OBHD; TRKB; DEE58; trk-B; EIEE58; GP145-TrkB; EC 2.7.10.1; Neurotrophic tyrosine kinase, receptor, type 2; BDNF-Tropomyosine receptor kinase B

EC NO.: 2.7.10.1

Applications: Human neurotrophic tyrosine kinase receptor type 2 (NTRK2) ELISA kit (93.75-6000 pg/mL) is used to quantify NTRK2 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of human, providing data to support research in a wide range of areas, including signal transduction, neurodevelopment, obesity, etc.

Research Area: Signal transduction; Neurodevelopment; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 93.75-6000 pg/mL

Sensitivity: 56.25 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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