Human neuroendocrine secretory protein 55 (NESP55) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-237

Assay Type: Quantitative competitive ELISA

Target Species: Human

Assay Target: NESP55

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Human neuroendocrine secretory protein 55 (NESP55) ELISA kit-Quantitative competitive

INQUIRY Datasheet MSDS

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Product Overview

Description: Human neuroendocrine secretory protein 55 (NESP55) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of NESP55 in human samples with a range of 0.5-10 ng/mL and a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The NESP55 ELISA kit utilizes a competitive enzyme immunoassay technique featuring a monoclonal anti-NESP55 antibody and an NESP55-HRP conjugate. Samples and assay buffer are incubated with the NESP55-HRP conjugate in a pre-coated plate for one hour. During this period, endogenous NESP55 from the sample competes with the NESP55-HRP conjugate for a limited number of antibody binding sites. After the incubation, the wells are washed five times. Subsequently, an HRP substrate is added, forming a blue complex that turns yellow upon the addition of a stop solution. The absorbance is measured at 450 nm. The color intensity is inversely proportional to the NESP55 concentration. A standard curve is generated to relate the O.D. to standard concentrations, from which the NESP55 levels in each sample are interpolated.

Background: NESP55 is a protein expressed in multiple neuroendocrine tissues, particularly in the pancreas, adrenal medulla, and hypothalamus. It belongs to a protein family known as NESP, whose members are genetically closely linked and whose expression is regulated by a specific epigenetic mechanism—imprinted genes. As an imprinted gene product, its expression is influenced by parental origin and may serve as a potential regulator of energy balance, obesity, and others.

Synonyms: GNAS; AHO; GSA; GSP; POH; GPSA; NESP; GNAS1; PHP1A; PHP1B; PHP1C; C20orf45

Applications: Human neuroendocrine secretory protein 55 (NESP55) ELISA kit is used to quantify NESP55 in serum, plasma, cell culture supernatants, body fluid, and tissue homogenate of human, providing data to support research in a wide range of areas, including endocrinology, epigenetic regulation, obesity, and others.

Research Area: Endocrinology; Epigenetic regulation; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 0.5-10 ng/mL

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 92-101%

Storage: Store at 2-8°C.

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