Human leptin receptor (LEPR) ELISA kit (0.5-10 ng/mL)

Cat. No.: 0126WXX-969

Assay Type: Quantitative competitive ELISA

Target Species: Human

Assay Target: LEPR

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Human leptin receptor (LEPR) ELISA kit (0.5-10 ng/mL)

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Product Overview

Description: Human leptin receptor (LEPR) ELISA kit (0.5-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEPR in human samples with a range of 0.5-10 ng/mL and a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microplate is pre-coated with a monoclonal antibody specific for LEPR. Standards or samples are added to the wells, allowing any LEPR to bind to the immobilized antibody. To quantify the target, a standardized HRP-conjugated polyclonal antibody specific for LEPR is added to "sandwich" the captured protein. After incubation and a thorough wash, substrate solutions are added. The HRP-substrate reaction produces a blue color that turns yellow upon adding sulphuric acid. The color intensity, measured at 450 nm, is used to interpolate the LEPR concentration from a standard curve.

Background: LEPR, as a peptide hormone receptor, is responsible for receiving leptin signals. When leptin binds to LEPR, it initiates a series of intracellular cascades, including the positive regulation of the JAK-STAT signaling pathway and the positive regulation of the PI3K signaling pathway. These pathways are crucial for translating leptin signals into biological responses, such as appetite suppression and metabolic regulation. The relationship between LEPR and obesity is paramount. When leptin functions as the “satiety hormone,” LEPR acts as the “satiety switch” in the brain and other tissues. Without functional receptors, leptin signals cannot be received regardless of how high leptin concentrations become.

Synonyms: CD295; LEPR; OBR; LR

Applications: Human leptin receptor (LEPR) ELISA kit (0.5-10 ng/mL) is used to quantify LEPR in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of human, providing data to support research in a wide range of areas, including signal transduction, CD & adhesion molecule, metabolic pathway, endocrinology, obesity, etc.

Research Area: Signal transduction; CD & Adhesion molecule; Metabolic pathway; Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 0.5-10 ng/mL

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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