Human leptin (LEP) ELISA kit (23.5 pg/mL)

Cat. No.: 0126WXX-808

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: LEP

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Human leptin (LEP) ELISA kit (23.5 pg/mL)

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Product Overview

Description: Human leptin (LEP) ELISA kit (23.5 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEP in human samples with a minimum detectable dose (sensitivity) of 23.5 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: The ELISA analytical biochemical technique is based on LEP antibody-LEP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LEP antigen targets in samples.

Background: LEP is a hormone that plays a crucial role in regulating body fat. Secreted by fat cells, it functions as part of a signaling pathway that controls the extent of fat storage in the human body. This protein belongs to the leptin family and possesses dual functions as both a growth factor and a hormone. LEP's primary functions include: regulating food intake, controlling energy expenditure, participating in glucose homeostasis regulation, modulating adipocyte differentiation, and influencing fatty acid metabolism. Obesity research frequently focuses on deciphering the mechanisms of leptin resistance and developing countermeasures, including the development of drugs that enhance brain sensitivity to leptin or the exploration of alternative pathways for regulating appetite and energy balance. Understanding leptin-mediated signaling pathways is central to this research.

Synonyms: OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

Applications: Human leptin (LEP) ELISA kit (23.5 pg/mL) is used to quantify LEP in human tissue homogenates, cell lysates, and other biological fluids of human, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, cardiovascular biology, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Cardiovascular biology; Obesity

Specification

Sample Type: Human Tissue homogenates; Cell lysates; Other biological fluids

Sensitivity: 23.5 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C.

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