Human leptin (LEP) ELISA kit (0.05-10 ng/mL)

Cat. No.: 0126WXX-813

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: LEP

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Human leptin (LEP) ELISA kit (0.05-10 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Human leptin (LEP) ELISA kit (0.05-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEP in human samples with a range of 0.05-10 ng/mL and a minimum detectable dose (sensitivity) of 0.021 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes a sandwich ELISA. The microplate is pre-coated with a human LEP capture antibody. Human LEP in the sample binds to the immobilized antibody, followed by the addition of a biotinylated human LEP detection antibody. Streptavidin-HRP is then introduced to bind the biotinylated complex. Following incubation and a wash step to remove unbound reagents, a substrate solution is added, initiating color development in direct proportion to the concentration of human LEP. The reaction is halted with an acidic stop solution, and absorbance is measured at 450 nm.

Background: LEP is a hormone that plays a crucial role in regulating body fat. Secreted by fat cells, it functions as part of a signaling pathway that controls the extent of fat storage in the human body. This protein belongs to the leptin family and possesses dual functions as both a growth factor and a hormone. LEP's primary functions include: regulating food intake, controlling energy expenditure, participating in glucose homeostasis regulation, modulating adipocyte differentiation, and influencing fatty acid metabolism. Obesity research frequently focuses on deciphering the mechanisms of leptin resistance and developing countermeasures, including the development of drugs that enhance brain sensitivity to leptin or the exploration of alternative pathways for regulating appetite and energy balance. Understanding leptin-mediated signaling pathways is central to this research.

Synonyms: OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

Applications: Human leptin (LEP) ELISA kit (0.05-10 ng/mL) is used to quantify LEP in serum, plasma, cell culture supernatant, cell lysates, and tissue homogenates of human, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, cardiovascular biology, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Cardiovascular biology; Obesity