Mouse hormone sensitive lipase (LIPE) ELISA kit (0.3-90 ng/mL)

Cat. No.: 0126WXX-423

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: LIPE

Size:

Mouse hormone sensitive lipase (LIPE) ELISA kit (0.3-90 ng/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Mouse hormone sensitive lipase (LIPE) ELISA kit (0.3-90 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LIPE in mouse samples with a range of 0.3-90 ng/mL and a minimum detectable dose (sensitivity) of 0.153 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This ELISA features a plate pre-coated with a mouse LIPE antibody. LIPE in the sample binds to the immobilized antibodies, followed by the addition of a biotinylated mouse LIPE antibody. Streptavidin-HRP is then introduced to bind the biotinylated antibody. After a wash step to remove unbound reagents, a substrate solution is added, and color develops in direct proportion to the amount of mouse LIPE present. The reaction is terminated with an acidic stop solution, and the absorbance is measured at 450 nm.

Background: LIPE, as a rate-limiting lipolytic enzyme, plays a pivotal role in lipid metabolism. It primarily hydrolyzes triglycerides stored in adipose tissue and cardiac muscle, converting them into free fatty acids. LIPE activity undergoes precise hormonal regulation: it is rapidly activated via cAMP-dependent phosphorylation under catecholamine influence, while insulin promotes its dephosphorylation and inactivation. Additionally, LIPE functions in steroid-producing tissues, converting cholesterol esters into cholesterol for steroid hormone synthesis. LIPE plays a pivotal role in the onset and progression of obesity, serving as the key rate-limiting enzyme in lipolysis. Its core function is to promote fat breakdown, releasing triglycerides stored within adipocytes for the body to utilize as energy. Obesity is frequently associated with abnormal regulation of LIPE activity within fat cells. Imbalances in its activity and regulation constitute a major molecular mechanism underlying fat accumulation and obesity.

Synonyms: HSL; LHS; Lipase, hormone sensitive; EC 3.1.1.79

EC NO.: 3.1.1.79

Applications: Mouse hormone sensitive lipase (LIPE) ELISA kit (0.3-90 ng/mL) is used to quantify LIPE in serum, plasma, cell culture supernates, cell lysates, and tissue homogenates of mouse, providing data to support research in a wide range of areas, including metabolism, obesity, and others.

Research Area: Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernates; Cell lysates; Tissue homogenates

Detection Range: 0.3-90 ng/mL

Sensitivity: 0.153 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out