Human insulin (INS) ELISA kit (2.5-50 mIU/mL)

Cat. No.: 0126WXX-581

Assay Type: Quantitative ELISA

Target Species: Human

Assay Target: INS

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Human insulin (INS) ELISA kit (2.5-50 mIU/mL)

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Product Overview

Description: Human insulin (INS) ELISA kit (2.5-50 mIU/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in human samples with a range of 2.5-50 mIU/mL and a minimum detectable dose (sensitivity) of 1.0 mIU/mL. The kit is highly sensitive and easy to use.

Assay Principle: This INS ELISA kit applies a quantitative sandwich enzyme immunoassay technique. The microtiter plate is pre-coated with a monoclonal antibody specific for INS to capture the target analyte. A standardized preparation of HRP-conjugated polyclonal antibody is then added to form a "sandwich" with the immobilized INS. Following incubation and thorough washing, substrate solutions are added to trigger a color change in positive wells. The reaction is terminated with sulphuric acid, and the INS concentration is interpolated from a standard curve based on the O.D. measured at 450 nm.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Human insulin (INS) ELISA kit (2.5-50 mIU/mL) is used to quantify INS in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of human, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 2.5-50 mIU/mL

Sensitivity: 1.0 mIU/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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