Human insulin (INS) CLIA kit

Cat. No.: 0126WXX-523

Assay Type: Quantitative competitive CLIA

Target Species: Human

Assay Target: INS

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INQUIRY Datasheet MSDS

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Product Overview

Description: Human insulin (INS) CLIA kit is a CLIA-based in vitro research tool designed specifically for the quantitative detection of INS in human samples with a range of 39.1-10000 pg/mL and a minimum detectable dose (sensitivity) of 15.1 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes a competitive inhibition chemiluminescence immunoassay where the microplate is pre-coated with a monoclonal antibody specific to INS. Endogenous INS from samples or standards competes with biotin-labeled INS for the limited binding sites on the immobilized antibody. Following the removal of unbound conjugates, Avidin-conjugated HRP is added. Upon addition of a chemiluminescent substrate, the intensity of the generated light emission is inversely proportional to the INS concentration in the sample.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Human insulin (INS) CLIA kit is used to quantify INS in serum, plasma, cell culture supernatants, and other biological fluids of human, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity