Human glucagon (GCG) ELISA kit (31.2-2000 pg/mL)

Cat. No.: 0126WXX-63

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: GCG

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Human glucagon (GCG) ELISA kit (31.2-2000 pg/mL)

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Product Overview

Description: Human glucagon (GCG) ELISA kit (31.2-2000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GCG in human samples with a range of 31.2-2000 pg/mL and a minimum detectable dose (sensitivity) of up to 12 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: A monoclonal capture antibody specific to Human GCG has been pre-coated onto the microplate. Samples are added, allowing the GCG to be immobilized. After a wash step, a biotinylated polyclonal antibody is added as the detection antibody. Following another wash, an avidin-peroxidase conjugate is introduced to bind the biotinylated antibody. A final wash removes all unbound reagents before adding TMB substrate. The HRP enzyme catalyzes a reaction producing a blue color, which turns yellow upon the addition of a stop solution (color reagent C). The final intensity is directly proportional to the quantity of GCG in the sample.

Background: Glucagon is a hormone secreted by A cells of the islets of Langerhans, playing a pivotal role in glucose metabolism and homeostasis. Its primary function is to regulate blood glucose levels by promoting gluconeogenesis and inhibiting glycolysis. The dynamic equilibrium between glucagon and insulin is crucial for maintaining healthy metabolism. The detection of glucagon and analysis of its associated pathways provide key insights into understanding and treating obesity.

Synonyms: GLP1; GLP2; GRPP; Glicentin-related polypeptide; Glucagen; Oxyntomodulin; Incretin hormone; GC

Applications: Human glucagon (GCG) ELISA kit (31.2-2000 pg/mL) is used to quantify GCG in human serum, plasma, and cell culture supernatant of human, providing data to support research in a wide range of areas, including endocrinology, obesity, metabolism, and others.

Research Area: Endocrinology; Obesity; Metabolism

Specification

Sample Type: Human serum; Plasma; Cell culture supernatant

Detection Range: 31.2-2000 pg/mL

Sensitivity: Up to 12 pg/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 70-110%

Storage: Store at 2-8°C.

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