Human insulin (INS) ELISA kit (39-2500 pg/mL)

Cat. No.: 0126WXX-574

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: INS

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Human insulin (INS) ELISA kit (39-2500 pg/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Human insulin (INS) ELISA kit (39-2500 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in human samples with a range of 39-2500 pg/mL and a minimum detectable dose (sensitivity) of <14 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes a sandwich immunoassay technique where the microtiter plate is pre-coated with an INS-specific antibody. Standards or samples are added to the wells along with a biotin-conjugated anti-INS antibody and avidin-HRP. Following incubation and the addition of TMB substrate, wells containing the INS complex exhibit a color change. The reaction is terminated with sulphuric acid, and the O.D. is measured spectrophotometrically at 450 nm. The concentration of INS is determined by comparing sample O.D. values to a standard curve.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Human insulin (INS) ELISA kit (39-2500 pg/mL) is used to quantify INS in human serum, plasma, cell culture supernatants, and other biological fluids of human, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Human serum; Plasma; Cell culture supernatants; Other biological fluids

Detection Range: 39-2500 pg/mL

Sensitivity: <14 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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