Rat adiponectin (ADP) ELISA kit (15.625-1000 pg/mL)

Cat. No.: OB0525WXX-287

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: ADP

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Rat adiponectin (ADP) ELISA kit (15.625-1000 pg/mL)

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Product Overview

Description: Rat adiponectin (ADP) ELISA kit (15.625-1000 pg/mL) is a ready-to-use ELISA kit for analyzing rat ADP levels with a detection range of 15.625-1000 pg/mL and a sensitivity of 9.375 pg/mL.

Assay Principle: The kit is based on a sandwich ELISA technique. The anti-ADP/Acrp30 antibody is pre-coated on a 96-well plate. Biotin-conjugated anti-ADP/Acrp30 antibody is then used as the detection antibody. Standards, test samples, and biotin-conjugated detection antibody are subsequently added to the wells and washed with buffer. HRP-streptavidin is added, and the unbound conjugate is washed away with washing solution. TMB substrate is used to observe the HRP enzyme reaction. TMB produces a blue product catalyzed by HRP, which turns yellow upon addition of an acidic termination solution. The density of the yellow color is proportional to the amount of ADP sample captured in the plate. The concentration of ADP can be calculated by reading the absorbance at 450nm with a microplate reader.

Background: ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.

Synonyms: ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin

Applications: Rat adiponectin (ADP) ELISA kit (15.625-1000 pg/mL) is designed for the in vitro quantitative analysis of ADP levels in rat serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Research Area: Hormone research; Lipid metabolism; Cell differentiation; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 15.625-1000 pg/mL

Sensitivity: 9.375 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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