Human estrogen receptor (ER) ELISA kit (1.0-25 ng/mL)

Cat. No.: OB0625WXX-692

Assay Type: Quantitative competitive ELISA

Target Species: Human

Assay Target: ER

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Human estrogen receptor (ER) ELISA kit (1.0-25 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Human estrogen receptor (ER) ELISA kit (1.0-25 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of ER in human with a range of 1.0-25 ng/mL and a specificity of 1.0 pg/mL.

Assay Principle: The kit utilizes a monoclonal anti-ER antibody and ER-HRP conjugate for detection. The assay sample and buffer are incubated with the ER-HRP conjugate for one hour on a pre-coated plate. At the end of the incubation period, the wells are decanted and washed five times. The wells are then incubated with substrate for the HRP enzyme. The product of the reaction of the enzyme with the substrate forms a blue colored complex. Finally, the reaction is stopped by the addition of a stop solution, which turns the solution yellow. The color intensity is measured spectrophotometrically at 450 nm on a microplate reader. The color intensity is inversely proportional to the concentration of ER because the ER in the sample competes with the ER-HRP conjugate for the anti-ER antibody binding site. Since there are a limited number of binding sites, as more sites are occupied by ER in the sample, fewer sites remain to bind the ER-HRP conjugate. A standard curve is plotted between color intensity (O.D.) and standard concentration. The concentration of ER in each sample can be extrapolated from this standard curve.

Background: ER is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Estrogens and their receptors play roles in sexual development, reproductive functions, bone, and other tissues. ERs are associated with various pathological processes.

Synonyms: Estradiol receptor; Oestrogen receptor; Receptors, Estrogen

Applications: Human estrogen receptor (ER) ELISA kit (1.0-25 ng/mL) is used to quantify ER in serum, plasma, cell culture supernatants, body fluid, tissue homogenate samples of human, providing data to support a wide range of studies.

Research Area: Endocrinology

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 1.0-25 ng/mL

Sensitivity: 1.0 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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