Human ectonucleotide pyrophosphatase/phosphodiesterase-1 (ENPP1) ELISA kit (0.156-10 ng/mL, <0.062 ng/mL)

Cat. No.: OB0625WXX-641

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: ENPP1

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Human ectonucleotide pyrophosphatase/phosphodiesterase-1 (ENPP1) ELISA kit (0.156-10 ng/mL, <0.062 ng/mL)

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Product Overview

Description: Human ectonucleotide pyrophosphatase/phosphodiesterase-1 (ENPP1) ELISA kit (0.156-10 ng/mL, <0.062 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of ENPP1 in human with a range of 0.156-10 ng/mL and a specificity of <0.062 ng/mL.

Assay Principle: The microtiter plates provided in this kit are pre-coated with an antibody specific for ENPP1. Standards or samples are then added to the appropriate microtiter plate wells along with the biotin-conjugated specific ENPP1 antibody preparation. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microplate well and incubated. Upon addition of TMB substrate solution, only wells containing ENPP1, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The OD of the sample is then compared to a standard curve to determine the concentration of ENPP1 in the sample.

Background: ENPP1 is a member of the ENPP family, which has broad specificity and cleaves various substrates. It is primarily involved in ATP hydrolysis at the plasma membrane, playing a role in regulating pyrophosphate levels, bone mineralization, and soft tissue calcification.

Synonyms: Membrane component chromosome 6 surface marker 1; Alkaline phosphodiesterase I; Plasma-cell membrane glycoprotein PC-1; M6S1; NPP1; NPPS; PC-1; PCA1; PDNP1

Applications: Human ectonucleotide pyrophosphatase/phosphodiesterase-1 (ENPP1) ELISA kit (0.156-10 ng/mL, <0.062 ng/mL) is used to quantify ENPP1 in human serum, plasma, tissue homogenates, and other biological fluid samples of human, providing data to support a wide range of studies.

Research Area: Bone mineralization and soft tissue calcification research; Obesity research