Human agouti-related protein (AGRP) ELISA kit (31.2-2000 pg/mL, 12.2 pg/mL)

Cat. No.: OB0525WXX-378

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: AGRP

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Human agouti-related protein (AGRP) ELISA kit (31.2-2000 pg/mL, 12.2 pg/mL)

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Product Overview

Description: Human agouti-related protein (AGRP) ELISA kit (31.2-2000 pg/mL, 12.2 pg/mL) is a ready-to-use ELISA kit for analyzing human AGRP levels with a detection range of 31.2-2000 pg/mL and a sensitivity of 12.2 pg/mL.

Assay Principle: The detection principle used in this kit is a sandwich enzyme immunoassay. The microtiter plate provided in the kit is pre-coated with an antibody specific for AGRP. Standards or samples are then added to the appropriate wells of the microtiter plate, followed by the addition of a biotin-conjugated antibody specific to AGRP. Avidin conjugated to HRP is then added to each microtiter plate well and incubated. Only wells containing AGRP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change upon addition of TMB substrate solution. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is then measured spectrophotometrically at 450 nm ± 10 nm. The concentration of AGRP in the sample can then be determined by comparing the OD of the sample to a standard curve.

Background: AGRP plays a role in body weight homeostasis, is involved in the control of eating behavior through the central melanocortin system, and acts as an inverse agonist of MC3R and MC4R, thereby inhibiting their constitutive activity. Genetic variants in AGRP may be responsible for obesity.

Synonyms: Agouti-related peptide; ASIP2

Applications: Human agouti-related protein (AGRP) ELISA kit (31.2-2000 pg/mL, 12.2 pg/mL) is designed for the in vitro quantitative analysis of AGRP levels in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples.

Research Area: Hormone research; Eating behavior; Signaling pathway; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 31.2-2000 pg/mL

Sensitivity: 12.2 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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