Guinea pig fatty acid synthase (FAS) ELISA kit (50-1000 μg/mL)

Cat. No.: OB0625WXX-835

Assay Type: Quantitative sandwich ELISA

Target Species: Guinea pig

Assay Target: FAS

Size:

Guinea pig fatty acid synthase (FAS) ELISA kit (50-1000 μg/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Guinea pig fatty acid synthase (FAS) ELISA kit (50-1000 μg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FAS in guinea pig with a range of 50-1000 μg/mL and a specificity of 1.0 μg/mL.

Assay Principle: Assay samples and buffer are incubated with FAS-HRP conjugate in pre-coated plates for one hour. At the end of the incubation, the sample is decanted, and the wells are washed five times. The wells are then incubated with HRP enzyme substrate. The product of the enzyme-substrate reaction forms a blue color complex. Finally, the reaction is stopped by adding a stop solution, which turns the solution yellow. Color intensity is measured spectrophotometrically at 450 nm on a microplate reader. The color intensity is inversely proportional to the FAS concentration because the FAS in the sample competes with the FAS-HRP conjugate for the binding site of the anti-FAS antibody. Since there are a limited number of binding sites, as more sites are occupied by FAS in the sample, fewer sites will bind to the FAS-HRP conjugate. A standard curve is plotted to relate the color intensity (O.D.) to the concentration of the standard. The concentration of FAS in each sample is inferred from this standard curve.

Background: FAS is a multi-enzyme protein that catalyzes fatty acid synthesis. It is essential for the de novo synthesis of long-chain saturated fatty acids from acetyl-CoA, malonyl-CoA, and NADPH. Studies have found that FAS may be involved in obesity by regulating feeding behavior and energy homeostasis, potentially playing a role in body weight regulation and obesity development.

Synonyms: Short chain dehydrogenase/reductase family 27X, Member 1; S-acetyltransferase; 3-oxoacyl synthase; Oleoyl hydrolase; Enoyl-acyl-carrier-protein reductase; OA519; SDR27X1; EC 2.3.1.85

EC NO.: 2.3.1.85

Applications: Guinea pig fatty acid synthase (FAS) ELISA kit (50-1000 μg/mL) is used to quantify FAS in serum, plasma, cell culture supernatants, body fluid, tissue homogenate samples of guinea pig, providing data to support a wide range of studies.

Research Area: Fatty acid synthesis; Lipid metabolism; Obesity research

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 50-1000 μg/mL

Sensitivity: 1.0 μg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out