Human adenosine deaminase-like protein (ADAL) ELISA kit (0.313-20 ng/mL, 0.188 ng/mL)

Cat. No.: OB0525WXX-200

Assay Type: Quantitative competitive ELISA

Target Species: Human

Assay Target: ADAL

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Human adenosine deaminase-like protein (ADAL) ELISA kit (0.313-20 ng/mL, 0.188 ng/mL)

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Product Overview

Description: Human adenosine deaminase-like protein (ADAL) ELISA kit (0.313-20 ng/mL, 0.188 ng/mL) is a ready-to-use ELISA kit for analyzing human ADAL levels with a detection range of 0.313-20 ng/mL and a sensitivity of 0.188 ng/mL.

Assay Principle: This ELISA kit utilizes a competitive ELISA method. The microtiter plate provided in this kit is pre-coated with an antibody specific for human ADAL. During the reaction, the human ADAL in the sample or standard competes with a fixed amount of biotin-labeled human ADAL for sites on the pre-coated monoclonal antibody specific for human ADAL. Excess binders and unbound samples or standards are washed off the plate, and then HRP-streptavidin is added to each microtiter well and incubated. TMB substrate solution is then added to each well. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm. The OD of the sample is then compared to a standard curve to determine the concentration of human ADAL in the sample.

Background: ADAL exhibits deamination activity towards N6-substituted purine nucleotide analogs and plays a role in purine metabolism.

Synonyms: Adenosine deaminase-like protein; EC 3.5.4.-

Applications: Human adenosine deaminase-like protein (ADAL) ELISA kit (0.313-20 ng/mL, 0.188 ng/mL) is designed for the in vitro quantitative analysis of ADAL levels in human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Research Area: Metabolism; Cell growth; Immunomodulation; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 0.313-20 ng/mL

Sensitivity: 0.188 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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