Human 6 phosphofructo 2 kinase/fructose 2; 6 biphosphatase 3 (PFKFB3) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-1260

Assay Type: Quantitative competitive ELISA

Target Species: Human

Assay Target: PFKFB3

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Human 6 phosphofructo 2 kinase/fructose 2; 6 biphosphatase 3 (PFKFB3) ELISA kit-Quantitative competitive

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Product Overview

Description: Human 6 phosphofructo 2 kinase/fructose 2; 6 biphosphatase 3 (PFKFB3) ELISA kit-Quantitative competitive is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PFKFB3 in human samples with a range of 5.0-100 ng/mL and a minimum detectable dose (sensitivity) of 1.0 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The ELISA analytical biochemical technique is based on PFKFB3 antibody-PFKFB3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PFKFB3 antigen targets in samples.

Background: PFKFB3 is a key bifunctional enzyme responsible for both the synthesis and degradation of fructose-2,6-bisphosphate (the most potent allosteric activator of the glycolytic rate-limiting enzyme PFK-1). Consequently, its activity is one of the primary determinants of cellular glycolytic flux. Given its pivotal role in glycolysis, PFKFB3 plays a significant role in diseases such as obesity.

Synonyms: IPFK2; PFK2; iPFK-2, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3

Applications: Human 6 phosphofructo 2 kinase/fructose 2, 6 biphosphatase 3 (PFKFB3) ELISA kit-Quantitative competitive is used to quantify PFKFB3 in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of human, providing data to support research in a wide range of areas, including inflammation, metabolism, obesity, and others.

Research Area: Inflammation; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 5.0-100 ng/mL

Sensitivity: 1.0 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 92-101%

Storage: Store at 2-8°C.

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