Horse lipoprotein lipase (LPL) ELISA kit (31.2-1000 pg/mL)

Cat. No.: 0126WXX-946

Assay Type: Quantitative sandwich ELISA

Target Species: Horse

Assay Target: LPL

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Horse lipoprotein lipase (LPL) ELISA kit (31.2-1000 pg/mL)

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Product Overview

Description: Horse lipoprotein lipase (LPL) ELISA kit (31.2-1000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LPL in horse samples with a range of 31.2-1000 pg/mL and a minimum detectable dose (sensitivity) of 5.0 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: Purified horse LPL antibody is used to coat microelisa strip plate wells to create a solid-phase antibody. LPL and HRP-labeled LPL antibody are added to the wells to form an antibody-antigen-antibody-enzyme complex. After thorough washing, the TMB substrate solution is introduced, becoming blue via HRP enzymatic catalysis. The reaction is terminated with a sulphuric acid solution, and the color change is measured spectrophotometrically at 450 nm. The concentration of LPL in the samples is determined by comparing the O.D. of the samples to the standard curve.

Background: LPL is an enzyme belonging to the lipoprotein lipase family. Its primary function is to break down triglycerides (a type of fat) in the blood. Specifically, LPL hydrolyzes triglycerides present in circulating lipoproteins such as chylomicrons and very low-density lipoproteins (VLDL). The free fatty acids and monoglycerides released during this process can be utilized by cells to generate energy or stored for later use. LPL's direct relevance to obesity research lies in its pivotal role in fat storage and metabolism. Given its central function in lipid metabolism, LPL has emerged as a key biological factor in obesity studies.

Synonyms: Clearing factor; Clearing factor lipase; EC 3.1.1.34

EC NO.: 3.1.1.34

Applications: Horse lipoprotein lipase (LPL) ELISA kit (31.2-1000 pg/mL) is used to quantify LPL in serum, plasma, tissue homogenate, feces, and urine of horse, providing data to support research in a wide range of areas, including signal transduction, metabolism, obesity, etc.

Research Area: Signal Transduction; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenate; Feces; Urine

Detection Range: 31.2-1000 pg/mL

Sensitivity: 5.0 pg/mL

Precision (Intra-assay): CV<15%

Precision (Inter-assay): CV<15%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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