Hamster adiponectin (ADP) ELISA kit (24T; 48T; 96T)

Cat. No.: OB0525WXX-211

Assay Type: Quantitative sandwich ELISA

Target Species: Hamster

Assay Target: ADP

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Hamster adiponectin (ADP) ELISA kit (24T; 48T; 96T)

INQUIRY Datasheet MSDS

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Product Overview

Description: Hamster adiponectin (ADP) ELISA kit (24T; 48T; 96T) is a ready-to-use ELISA kit for analyzing hamster ADP levels with a detection range of 0.469-30 ng/mL and a sensitivity of 0.117 ng/mL.

Assay Principle: The assay is performed using a quantitative sandwich enzyme immunoassay technique. An antibody specific for ADP has been pre-coated on a microtiter plate. Standards and samples are pipetted into the wells, and any ADP is bound by the immobilized antibody. After removal of unbound material, a biotin-conjugated antibody specific for ADP is added to the wells. After washing, avidin-conjugated HRP is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color develops in proportion to the amount of ADP bound in the initial step. The color development is stopped, and the color intensity is measured.

Background: ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.

Synonyms: ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin

Applications: Hamster adiponectin (ADP) ELISA kit (24T, 48T, 96T) is designed for the in vitro quantitative analysis of ADP levels in hamster serum, plasma, and tissue homogenate samples.

Research Area: Hormone research; Lipid metabolism; Cell differentiation; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates

Detection Range: 0.469-30 ng/mL

Sensitivity: 0.117 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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