Chicken C-reactive protein (CRP) high sensitive ELISA kit (0.156-10 ng/mL)

Cat. No.: OB0625WXX-435

Assay Type: Quantitative sandwich ELISA

Target Species: Chicken

Assay Target: CRP

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Chicken C-reactive protein (CRP) high sensitive ELISA kit (0.156-10 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Chicken C-reactive protein (CRP) high sensitive ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CRP in chicken with a range of 0.156-10 ng/mL and a specificity of <0.055 ng/mL.

Assay Principle: The microtiter plates provided in this kit are pre-coated with a CRP-specific antibody. Standards or samples are then added to the wells of the microtiter plate along with the CRP-specific biotin-conjugated antibody. Avidin conjugated to horseradish peroxidase (HRP) is then added to each well and incubated. Upon addition of the TMB substrate solution, only the wells containing CRP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is then measured spectrophotometrically at 450 nm ± 10 nm. The OD of the sample is then compared to a standard curve to determine the concentration of CRP in the sample.

Background: CRP is an acute-phase protein of hepatic origin, whose circulating concentrations rise in response to inflammation. It is considered a key plasma protein that can bind to Leptin, and its potential role in regulating leptin's function in obesity is crucial.

Synonyms: Pentraxin-related; pentraxin 1; C-RP; PTX1

Applications: Chicken C-reactive protein (CRP) high sensitive ELISA kit (0.156-10 ng/mL) is used to quantify CRP in serum, plasma, tissue homogenates, and other biological fluid samples of chicken, providing data to support a wide range of studies.

Research Area: Inflammatory reaction; Obesity research

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Other biological fluids

Detection Range: 0.156-10 ng/mL

Sensitivity: <0.055 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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