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Mouse angiotensin II type 1 receptor antibody (ATIIR1 Ab) ELISA kit
Cat. No.:
OB0625WXX-219
Assay Type:
Quantitative sandwich ELISA
Target Species:
Mouse
Assay Target:
ATIIR1 Ab
Size:
Add to basket
Product Overview
Description:
Mouse angiotensin II type 1 receptor antibody (ATIIR1 Ab) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of ATIIR1 Ab in mouse with a range of 0.312-20 ng/mL and a specificity of up to 0.06 ng/mL.
Assay Principle:
The pre-coated antigen is a related mouse ATIIR1 Ab antigen, and the detection antigen is another antigen. The samples are added to the ELISA plate wells and rinsed with PBS or TBS after being added to the wells individually. The avidin-peroxidase conjugate is then added sequentially to the wells. The enzyme conjugate is washed thoroughly with PBS or TBS and then colored with TMB substrate, which reacts with peroxidase activity to produce a blue product, which finally turns yellow upon addition of the termination solution. The intensity of the color is positively correlated with the amount of target analyte in the sample.
Background:
ATIIR1 Abs are considered to be potential effectors of acute and chronic antibody-mediated rejection.
Synonyms:
AT1R-Ab; AT1R antibody; Angiotensin II type-1 receptor antibody; Anti-AT1R antibody
Applications:
Mouse angiotensin II type 1 receptor antibody (ATIIR1 Ab) ELISA kit is used to quantify ATIIR1 Ab in mouse serum, plasma, and cell culture supernatant samples of mouse, providing data to support a wide range of studies.
Research Area:
Immune response
Specification
Sample Type:
Mouse serum; Plasma; Cell culture supernatant
Detection Range:
0.312-20 ng/mL
Sensitivity:
Up to 0.06 ng/mL
Precision (Intra-assay):
CV≤8%
Precision (Inter-assay):
CV≤12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.
