Guinea pig peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-1475

Assay Type: Quantitative sandwich ELISA

Target Species: Guinea pig

Assay Target: PPARα

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Guinea pig peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Guinea pig peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PPARα in guinea pig samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of <0.05 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This experiment employs a double-sandwich ELISA technique using a guinea pig PPARα monoclonal capture antibody and a biotin-labeled polyclonal detection antibody. Samples and the detection antibody are added to the plate and washed with PBS or TBS. Subsequently, avidin-peroxidase conjugates are added. After a thorough wash, TMB substrate is used for coloration, producing a blue product that turns yellow under acidic conditions. The color depth is positively correlated with the concentration of the target analyte in the samples.

Background: PPAR-alpha, a member of the NR1 nuclear hormone receptor subfamily located at chromosomal position 22q13.31, serves as a master ligand-activated transcription factor and a cornerstone regulator of lipid homeostasis in obesity research. Primarily localized within the nucleus, its transactivation activity—requiring heterodimerization with RXRA and being antagonized by NR2C2—is triggered by various endogenous ligands such as 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC), satiety-regulating lipids like oleylethanolamide, and exogenous peroxisome proliferators. Beyond its fundamental role in activating the peroxisomal beta-oxidation pathway through genes such as ACOX1 and P450, PPAR-alpha orchestrates a complex physiological network including fatty acid transport, lipoprotein metabolism, and the negative regulation of appetite and glycolysis. Its multifaceted molecular functions—ranging from zinc ion binding to steroid hormone-mediated signaling—integrate critical biological processes such as insulin stimulus response, gluconeogenesis, and circadian rhythm regulation, making it an essential target for understanding and intervening in metabolic disorders and chronic obesity.

Synonyms: Peroxisome proliferator activated receptor alpha; NR1C1; PPAR-A; HPPAR; Nuclear receptor subfamily 1 group C member 1; PPARa; PPARA; PPAR-alpha

Applications: Guinea pig peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.156-10 ng/mL) is used to quantify PPARα in guinea pig serum, plasma, and cell culture supernatant of guinea pig, providing data to support research in a wide range of areas, including signal transduction, developmental science, obesity, and others.

Research Area: Signal transduction; Developmental science; Obesity

Specification

Sample Type: Guinea pig serum; Plasma; Cell culture supernatant

Detection Range: 0.156-10 ng/mL

Sensitivity: <0.05 ng/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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