Guinea pig fatty acid synthase (FAS) ELISA kit (0.625-40 ng/mL)

Cat. No.: OB0625WXX-856

Assay Type: Quantitative sandwich ELISA

Target Species: Guinea pig

Assay Target: FAS

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Guinea pig fatty acid synthase (FAS) ELISA kit (0.625-40 ng/mL)

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Product Overview

Description: Guinea pig fatty acid synthase (FAS) ELISA kit (0.625-40 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FAS in guinea pig with a range of 0.625-40 ng/mL and a specificity of 0.375 ng/mL.

Assay Principle: The micro ELISA plates in this kit are pre-coated with an antibody specific for FAS. Standards or samples are added to the corresponding wells of the microtiter plate and bound to the specific antibody. The biotinylated detection antibody specific for FAS and the avidin-HRP conjugate are then added sequentially to each microplate well and incubated. The free components are washed away. Substrate solution is added to each well. Only wells containing FAS, biotinylated detection antibody, and avidin-HRP conjugate will appear blue. Upon addition of sulfuric acid solution, the enzyme-substrate reaction terminates, and the color changes to yellow. Optical density (OD) is measured spectrophotometrically at 450 nm ± 2 nm. The OD value is proportional to the concentration of FAS. The concentration of FAS in the sample is calculated by comparing the OD value of the sample with the standard curve.

Background: FAS is a multi-enzyme protein that catalyzes fatty acid synthesis. It is essential for the de novo synthesis of long-chain saturated fatty acids from acetyl-CoA, malonyl-CoA, and NADPH. Studies have found that FAS may be involved in obesity by regulating feeding behavior and energy homeostasis, potentially playing a role in body weight regulation and obesity development.

Synonyms: Short chain dehydrogenase/reductase family 27X, Member 1; S-acetyltransferase; 3-oxoacyl synthase; Oleoyl hydrolase; Enoyl-acyl-carrier-protein reductase; OA519; SDR27X1; EC 2.3.1.85

EC NO.: 2.3.1.85

Applications: Guinea pig fatty acid synthase (FAS) ELISA kit (0.625-40 ng/mL) is used to quantify FAS in serum, plasma, and biological fluid samples of guinea pigs, providing data to support a wide range of studies.

Research Area: Fatty acid synthesis; Lipid metabolism; Obesity research

Specification

Sample Type: Serum; Plasma; Biological fluids

Detection Range: 0.625-40 ng/mL

Sensitivity: 0.375 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C.

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