Chicken insulin (INS) ELISA kit (100-1600 pg/mL)

Cat. No.: 0126WXX-546

Assay Type: Quantitative competitive ELISA

Target Species: Chicken

Assay Target: INS

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Chicken insulin (INS) ELISA kit (100-1600 pg/mL)

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Product Overview

Description: Chicken insulin (INS) ELISA kit (100-1600 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in chicken samples with a range of 100-1600 pg/mL and a minimum detectable dose (sensitivity) of 100 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes a competitive inhibition enzyme immunoassay technique. The microtiter plate is pre-coated with an INS-specific antibody, and a competitive reaction is initiated between the INS in the sample (or standards) and a fixed amount of biotin-conjugated INS for the limited antibody binding sites. A higher concentration of INS in the sample results in less biotin-conjugated INS binding to the plate. Following a wash step, Avidin-conjugated HRP is added. The subsequent color development is inversely proportional to the amount of INS present in the sample.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Chicken insulin (INS) ELISA kit (100-1600 pg/mL) is used to quantify INS in serum, plasma, and tissue homogenates of chicken, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates

Detection Range: 100-1600 pg/mL

Sensitivity: 100 pg/mL

Precision (Intra-assay): CV<15%

Precision (Inter-assay): CV<15%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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