Chicken hydroxyacyl-coenzyme A dehydrogenase, mitochondrial (HADH) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-316

Assay Type: Quantitative competitive ELISA

Target Species: Chicken

Assay Target: HADH

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Chicken hydroxyacyl-coenzyme A dehydrogenase, mitochondrial (HADH) ELISA kit-Quantitative competitive

INQUIRY Datasheet MSDS

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Product Overview

Description: Chicken hydroxyacyl-coenzyme A dehydrogenase, mitochondrial (HADH) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of HADH in chicken samples with a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The HADH ELISA kit employs a competitive ELISA technique. Samples and assay buffer are incubated with an HADH-HRP conjugate in a pre-coated plate for one hour. During this time, endogenous HADH from the sample competes with the HADH-HRP conjugate for a limited number of antibody binding sites. After incubation, the wells are washed five times. An HRP substrate is then added, forming a blue complex that turns yellow upon the addition of a stop solution. The absorbance is measured at 450 nm. The color intensity is inversely proportional to the HADH concentration. A standard curve is generated to relate the O.D. to standard concentrations, from which the HADH levels in each sample are interpolated.

Background: HADH is a crucial redox enzyme in mitochondria that plays a central role in fatty acid β-oxidation. This enzyme is primarily responsible for the breakdown of short-chain fatty acids, and its abnormal activity can lead to various metabolic disorders. Given its function in fatty acid β-oxidation, HADH may also play a role in obesity.

Synonyms: Hydroxyacyl-CoA dehydrogenase; SCHAD; HADHSC; HADH1; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; EC 1.1.1.35; L-3-Hydroxyacyl-coenzyme A dehydrogenase, short chain; Short-chain 3-hydroxyacyl-CoA dehydrogenase; HCDH; HAD1; HAD; EC 1.1.1.35

EC NO.: 1.1.1.35

Applications: Chicken hydroxyacyl-coenzyme A dehydrogenase, mitochondrial (HADH) ELISA kit is used to quantify HADH in serum, plasma, cell culture supernatants, body fluid, and tissue homogenate of chicken, providing data to support research in a wide range of areas, including enzyme & kinase, metabolism, obesity, and others.

Research Area: Enzyme & kinase; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 0.5-10 ng/mL

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 92-103%

Storage: Store at 2-8°C.

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