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Chicken estrogen receptor α (ERα) ELISA kit

Cat. No.: OB0625WXX-733
Assay Type: Quantitative sandwich ELISA
Target Species: Chicken
Assay Target: ERα
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Chicken estrogen receptor α (ERα) ELISA kit
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Product Overview

Description: Chicken estrogen receptor α (ERα) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of ERα in chicken with a range of 3.125-200 pg/mL and a specificity of 1.875 pg/mL.
Assay Principle: The micro ELISA plates provided in this kit are pre-coated with an antibody specific to chicken ERα. Standards or samples are added to the appropriate micro ELISA plate wells and bound to the specific antibody. Biotinylated detection antibody specific for chicken ERα and avidin-horseradish peroxidase (HRP) conjugate are then added sequentially to each microplate well and incubated. Free components are washed away. Substrate solution is added to each well. Only wells containing chicken ERα, biotinylated detection antibody, and avidin-HRP conjugate will appear blue. Upon addition of sulfuric acid solution, the enzyme-substrate reaction terminates, and the color turns yellow. Optical density (OD) is measured spectrophotometrically at 450 nm ± 2 nm. The OD value is proportional to the concentration of chicken ERα. The concentration of Chicken ERα in the sample can be calculated by comparing the OD value with the standard curve.
Background: ERα, a member of the steroid receptor superfamily, contains highly conserved DNA-binding and ligand-binding domains. It is activated by the sex hormone estrogen and is involved in the physiological development and function of various organ systems.
Synonyms: Estrogen receptor alpha; ESR1; ERa; NR3A1; EC 3.1.1.3
Applications: Chicken estrogen receptor α (ERα) ELISA kit is used to quantify ERα in serum, plasma, and biological fluid samples of chicken, providing data to support a wide range of studies.
Research Area: Endocrinology

Specification

Sample Type: Serum; Plasma; Biological fluids
Detection Range: 3.125-200 pg/mL
Sensitivity: 1.875 pg/mL
Cross-reactivity: No significant cross-reactivity or interference was observed.
Storage: Store at 4°C.

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