Canine alpha-ketoglutarate-dependent dioxygenase (FTO) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-19

Assay Type: Quantitative competitive ELISA

Target Species: Canine

Assay Target: FTO

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Canine alpha-ketoglutarate-dependent dioxygenase (FTO) ELISA kit-Quantitative competitive

INQUIRY Datasheet MSDS

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Product Overview

Description: Canine alpha-ketoglutarate-dependent dioxygenase (FTO) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FTO in canine samples with a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The FTO ELISA kit utilizes a competitive enzyme immunoassay technique involving a polyclonal anti-FTO antibody and an FTO-HRP conjugate. Samples and assay buffer are incubated with the FTO-HRP conjugate in a pre-coated plate for one hour. During this time, endogenous FTO from the sample competes with the FTO-HRP conjugate for a limited number of antibody binding sites. After five washes, the wells are incubated with an HRP substrate, forming a blue complex that turns yellow upon the addition of a stop solution. The absorbance is measured at 450 nm. The color intensity is inversely proportional to the FTO concentration. A standard curve is generated to relate the optical density (O.D.) to standard concentrations, from which the FTO levels in each sample are interpolated.

Background: FTO is a crucial dioxygenase primarily responsible for repairing alkylated DNA and RNA through oxidative demethylation. It exhibits the highest activity toward single-stranded RNA containing 3-methyluracil and requires molecular oxygen, α-ketoglutarate, and iron as cofactors. Beyond its role in nucleic acid repair, FTO participates in regulating systemic metabolic rate, energy expenditure, and energy homeostasis, thereby influencing body composition and adipose tissue accumulation. Defects in this protein are associated with various disorders, including growth retardation, developmental delays, and premature mortality. The relationship between FTO and obesity represents a highly significant research area, given that FTO is currently one of the strongest known genetic associates of obesity. Research indicates that FTO influences body weight by regulating energy balance and fat accumulation. Its mechanisms involve modulating appetite and satiety signaling pathways, as well as influencing lipolysis and lipogenesis processes. Specific FTO gene variants have been demonstrated to be significantly associated with higher BMI and obesity risk.

Synonyms: GDFD; IFEX9; ALKBH9; BMIQ14; KIAA1752; Fat mass and obesity-associated protein; FTO, alpha-ketoglutarate dependent dioxygenase; EC 1.14.11.-

EC NO.: 1.14.11.-

Applications: Canine alpha-ketoglutarate-dependent dioxygenase (FTO) ELISA kit is used to quantify FTO in serum, plasma, cell culture supernatants, body fluid, and tissue homogenate of canine, providing data to support research in a wide range of areas, including enzyme & kinase, obesity, and others.

Research Area: Enzyme & kinase; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 0.5-10 ng/mL

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 94-103%

Storage: Store at 2-8°C.

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