Bovine leptin (LEP) ELISA kit (15.625-1000 pg/mL)

Cat. No.: 0126WXX-787

Assay Type: Quantitative sandwich ELISA

Target Species: Bovine

Assay Target: LEP

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Bovine leptin (LEP) ELISA kit (15.625-1000 pg/mL)

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Product Overview

Description: Bovine leptin (LEP) ELISA kit (15.625-1000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEP in porcine samples with a range of 15.625-1000 pg/mL and a minimum detectable dose (sensitivity) of 9.375 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is based on sandwich ELISA technology. An anti-LEP capture antibody is pre-coated onto 96-well plates. Standards, samples, and a biotin-conjugated anti-LEP detection antibody are added sequentially to the wells and washed with buffer. HRP-Streptavidin is then introduced, and unbound conjugates are removed. TMB substrate is used to visualize the HRP enzymatic reaction, producing a blue product that turns yellow upon adding an acidic stop solution. The intensity of the yellow color is directly proportional to the concentration of LEP captured, which is determined by measuring absorbance at 450 nm.

Background: LEP is a hormone that plays a crucial role in regulating body fat. Secreted by fat cells, it functions as part of a signaling pathway that controls the extent of fat storage in the human body. This protein belongs to the leptin family and possesses dual functions as both a growth factor and a hormone. LEP's primary functions include: regulating food intake, controlling energy expenditure, participating in glucose homeostasis regulation, modulating adipocyte differentiation, and influencing fatty acid metabolism. Obesity research frequently focuses on deciphering the mechanisms of leptin resistance and developing countermeasures, including the development of drugs that enhance brain sensitivity to leptin or the exploration of alternative pathways for regulating appetite and energy balance. Understanding leptin-mediated signaling pathways is central to this research.

Synonyms: OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

Applications: Bovine leptin (LEP) ELISA kit (15.625-1000 pg/mL) is used to quantify LEP in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of bovine, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, cardiovascular biology, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Cardiovascular biology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 15.625-1000 pg/mL

Sensitivity: 9.375 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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