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Vitamin B12 test kit

Bovine butyrylcholinesterase (BCHE) ELISA kit

Cat. No.: OB0625WXX-229
Assay Type: Quantitative sandwich ELISA
Target Species: Bovine
Assay Target: BCHE
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Bovine butyrylcholinesterase (BCHE) ELISA kit
INQUIRY Datasheet MSDS
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Product Overview

Description: Bovine butyrylcholinesterase (BCHE) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of BCHE in bovine with a range of 0.312-20 ng/mL and a specificity of up to 0.06 ng/mL.
Assay Principle: The pre-coated antibody is a bovine BCHE monoclonal antibody, and the detection antibody is a biotin-labeled polyclonal antibody. The samples and biotin-labeled antibodies are added to the ELISA plate wells and rinsed with PBS or TBS. Avidin-peroxidase conjugate is then sequentially added to the ELISA wells. The reactants are thoroughly rinsed with PBS or TBS and then colored with TMB substrate. TMB turns blue when catalyzed by peroxidase and finally turns yellow in the presence of acid. The shade of color is positively correlated with the detection factor in the sample.
Background: BCHE is a non-specific cholinesterase that has broad substrate specificity and is involved in detoxification reactions, drug metabolism, among other processes.
Synonyms: BuChE; CHE1; E1; Pseudocholinesterase; Cholinesterase; Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II; EC 3.1.1.8
EC NO.: 3.1.1.8
Applications: Bovine butyrylcholinesterase (BCHE) ELISA kit is used to quantify BCHE in bovine serum, plasma, cell culture supernatant samples of bovine, providing data to support a wide range of studies.
Research Area: Metabolic pathway

Specification

Sample Type: Bovine serum; Plasma; Cell culture supernatant
Detection Range: 0.312-20 ng/mL
Sensitivity: Up to 0.06 ng/mL
Precision (Intra-assay): CV≤8%
Precision (Inter-assay): CV≤12%
Cross-reactivity: No significant cross-reactivity or interference was observed.
Storage: Store at 2-8°C.

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