Bovine adiponectin (ADP) ELISA kit (0.62-40.0 μg/mL)

Cat. No.: OB0525WXX-208

Assay Type: Quantitative ELISA

Target Species: Bovine

Assay Target: ADP

Size:

Bovine adiponectin (ADP) ELISA kit (0.62-40.0 μg/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Bovine adiponectin (ADP) ELISA kit (0.62-40.0 μg/mL) is a ready-to-use ELISA kit for analyzing bovine ADP levels with a detection range of 0.62-40.0 μg/mL.

Assay Principle: The microtiter plate provided in this kit is pre-coated with a specific ADP antibody. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated ADP-specific polyclonal antibody preparation, and HRP-conjugated avidin is added to each microtiter plate well and incubated. TMB substrate solution is then added to each well. Only wells containing ADP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 2 nm. The concentration of ADP in the sample is then determined by comparing the OD of the sample to a standard curve.

Background: ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.

Synonyms: ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin

Applications: Bovine adiponectin (ADP) ELISA kit (0.62-40.0 μg/mL) is designed for the in vitro quantitative analysis of ADP levels in bovine serum, plasma, tissue homogenates, cell culture supernates, and other biological fluid samples.

Research Area: Hormone research; Lipid metabolism; Cell differentiation; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell culture supernates; Other biological fluids

Detection Range: 0.62-40.0 μg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out