AdipoUpX™ Human PPARGC1B Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PPARGC1B Lentiviral Particle (Overexpression) is a high-titer system for the specific and stable expression of the human PPARGC1B (PGC-1β) gene. As a coactivator of mitochondrial and thermogenic genes, its stable overexpression is an ideal tool for researching energy expenditure and lipid management. The lentivirus characteristic of genome integration establishes reliable cell models for long-term functional studies. Comprehensive quality control is performed on all particles to ensure safety, repeatability, and high-fidelity results.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: PPARGC1B

Full Name: PPARG coactivator 1 beta

Gene Symbol: PERC; ERRL1; PGC1B; PGC-1(beta)

Gene ID: 133522

RefSeq ID-1: NP_001166169.1

RefSeq ID-2: NM_001172698.2

Summary: PPARGC1B encodes a transcriptional coactivator that enhances the activity of nuclear receptors and transcription factors such as estrogen receptor alpha, NRF1, and glucocorticoid receptor. It participates in fat oxidation, glucose metabolism, and energy expenditure regulation. This gene shows reduced expression in prediabetic and type 2 diabetes patients, and certain variants are linked to obesity susceptibility. Three transcript variants encoding different isoforms have been reported.

AdipoUpX™ Human PPARGC1B Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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