AdipoUpX™ Human PIK3CA Ad5 Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PIK3CA Ad5 Adenoviral Particle (Overexpression) is engineered for efficient overexpression of the human PIK3CA gene, a key catalytic subunit in the PI3K/AKT signaling pathway. It allows for the rapid functional validation of lipid biosynthesis and glucose uptake mechanisms. This product is supported by flexible reporter gene selection and comprehensive quality control screening, including titer, sterility, and mycoplasma testing.

Production Cell Line: HEK293

Viral Backbone: Adenovirus type5 (dE1/E3)

Promoter: CMV

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: All viral preparations have been validated via PCR to confirm the integrity of their vector genomes.

Sterility Test: This product was confirmed to be sterile upon testing.

Mycoplasma Test: This product has passed quality control, confirming that it is free of mycoplasma contamination.

Other QC: We perform customized supplementary testing and in vitro/in vivo transduction assessments based on specific project requirements to analyze the expression levels and biological functions of transgenes.

Storage: Upon receipt of viral preparations, they must be immediately transferred to a -80°C environment for long-term storage.

Stability: The product guarantees excellent stability for 6-12 months when stored continuously at -80°C (and in some cases, up to 2 years). Upon thawing, the working solution is refrigerated at 4°C for up to 2-3 weeks without significant loss of biological activity.

Shipping Condition: Our adenovirus viral products are shipped using dry ice.

Handling Notes: Viral particles are highly sensitive to temperature fluctuations and freeze-thaw cycles. To maintain titer and avoid repeated freeze-thaw cycles, it is recommended to aliquot the vector immediately upon receipt into low-protein-binding tubes and store according to specified conditions. Ensure all operations are performed within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Our company is committed to product quality, and all products have undergone rigorous internal QC inspections. Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application. Users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements throughout all operational processes.

Target Profile

Gene Name: PIK3CA

Full Name: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha

Gene Symbol: HMH; MCM; CCM4; CWS5; MCAP; PI3K; CLAPO; CLOVE; MCMTC; PI3K-alpha; p110-alpha

Gene ID: 5290

RefSeq ID-1: NP_006209.2

RefSeq ID-2: NM_006218.4

Summary: PIK3CA encodes the catalytic subunit of phosphatidylinositol 3-kinase (PI3K), which phosphorylates phosphoinositides to regulate intracellular signaling. Although PIK3CA is well-known for its oncogenic potential, the PI3K pathway also influences insulin signaling, energy metabolism, and adipocyte function, suggesting a potential role in obesity. A pseudogene exists on chromosome 22.

AdipoUpX™ Human PIK3CA Ad5 Particle (Overexpression)

Product Overview

Specification

Target Profile

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