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LipoKnoxa™ Human IFT27 shRNA Ad5 Particle (Silencing)
Cat. No.:
V0126XX84
Species:
Human
Target Gene:
IFT27
Vector System:
Adenovirus
Modulation Type:
Silencing (shRNA)
SPECIFIC INQUIRY
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| Sub Cat. No. | TargetSeq | Region | Inquiry |
|---|---|---|---|
| V0126XX84-1 | TTCGGAAATGCTGGATAAATT | CDS | Inquiry |
| V0126XX84-2 | ACAGTGTGGAACTCTTCATTT | CDS | Inquiry |
| V0126XX84-3 | CCCTGACAACAGGAATGGATT | CDS | Inquiry |
| V0126XX84-4 | Other | Inquiry |
Product Overview
Description:
LipoKnoxa™ Human IFT27 shRNA Ad5 Particle (Silencing) is engineered to silence IFT27, a GTPase required for BBSome entry into the primary cilium. Silencing this gene is essential for researching ciliopathy-related obesity and the trafficking of metabolic receptors. Each viral preparation is subjected to exhaustive quality control—including functional activity and sequence verification—offering a secure foundation for cellular and molecular biology data.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type 5 (dE1/E3)
Promoter:
U6; CMV; EF1α; CAG; UBC
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations are validated via Sequencing and PCR to ensure 100% sequence identity and the structural integrity of the vector genomes.
Sterility Test:
This product has been certified sterile following comprehensive microbial growth analysis, confirming the absence of bacterial and fungal contamination.
Mycoplasma Test:
This product was certified negative for mycoplasma contamination following stringent QC analysis, ensuring the absence of all mycoplasmal agents.
Other QC:
Beyond standard protocols, we offer customized knockdown efficiency validation through in vitro and in vivo assessments. This includes precise analysis of mRNA/protein reduction and subsequent biological responses to ensure the functional potency of the shRNA-mediated gene silencing.
Storage:
Upon receipt, viral preparations should be immediately transferred to -80°C for long-term storage to ensure maximum stability and maintain product integrity.
Stability:
This product maintains excellent biological activity for 6–12 months (and up to 2 years in specific cases) when stored continuously at -80°C. Once thawed, the working solution remains stable for 2–3 weeks at 4°C without significant loss of viral potency.
Shipping Condition:
All viral preparations are shipped on dry ice to ensure maximum biological activity and stability during transit.
Handling Notes:
Viral particles are susceptible to temperature fluctuations and freeze-thaw cycles. To preserve functional titers, it is essential to aliquot the vector into low-protein-binding tubes immediately upon first thaw. To ensure experimental success and biological safety, all procedures must be conducted within a certified biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
While our products are committed to excellence through rigorous internal QC inspections, we cannot guarantee specific performance or experimental outcomes due to the inherent complexity of biological systems. Users assume full responsibility for product storage, handling, and strict compliance with all applicable safety protocols, biosafety requirements, and legal regulations during all operational processes.
Target Profile
Gene Name:
IFT27
Full Name:
Intraflagellar transport 27
Gene Symbol:
RAYL; BBS19; RABL4; FAP156; CFAP156
Gene ID:
11020
RefSeq ID-1:
NP_001171172.1
RefSeq ID-2:
NM_001177701.3
Summary:
IFT27 encodes a GTP-binding protein that serves as a fundamental component of the intraflagellar transport (IFT) complex B. This complex is responsible for the movement of cargo within the primary cilium, the cell's sensory antenna. Recent research highlights IFT27 as a link between ciliary protein trafficking and cell cycle control. Because functional cilia are required for the brain to sense metabolic hormones (like leptin), disruptions in IFT27-mediated trafficking are a recognized cause of ciliopathy-associated obesity.
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