Product Overview
Description:
AdipoUpX™ Human INPP5E Ad5 Adenoviral Particle (Overexpression) enables the efficient transient overexpression of the human MAPK14 (p38 alpha) gene. This kinase is central to stress-activated signaling and the "browning" of white adipose tissue. This product serves as a powerful tool for in vitro functional validation of thermogenic activation pathways. Product quality is guaranteed by rigorous purification and titer validation.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type5 (dE1/E3)
Promoter:
CMV
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations have been validated via PCR to confirm the integrity of their vector genomes.
Sterility Test:
This product was confirmed to be sterile upon testing.
Mycoplasma Test:
This product has passed quality control, confirming that it is free of mycoplasma contamination.
Other QC:
We perform customized supplementary testing and in vitro/in vivo transduction assessments based on specific project requirements to analyze the expression levels and biological functions of transgenes.
Storage:
Upon receipt of viral preparations, they must be immediately transferred to a -80°C environment for long-term storage.
Stability:
The product guarantees excellent stability for 6-12 months when stored continuously at -80°C (and in some cases, up to 2 years). Upon thawing, the working solution is refrigerated at 4°C for up to 2-3 weeks without significant loss of biological activity.
Shipping Condition:
Our adenovirus viral products are shipped using dry ice.
Handling Notes:
Viral particles are highly sensitive to temperature fluctuations and freeze-thaw cycles. To maintain titer and avoid repeated freeze-thaw cycles, it is recommended to aliquot the vector immediately upon receipt into low-protein-binding tubes and store according to specified conditions. Ensure all operations are performed within a biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Our company is committed to product quality, and all products have undergone rigorous internal QC inspections. Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application. Users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements throughout all operational processes.
Target Profile
Gene Name:
MAPK14
Full Name:
Mitogen-activated protein kinase 14
Gene Symbol:
RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Summary:
MAPK14 encodes a stress-activated member of the mitogen-activated protein kinase (MAPK) family that serves as a central signaling node integrating diverse biochemical inputs. MAPK14 is activated in response to environmental stressors and pro-inflammatory cytokines through phosphorylation by upstream MAP kinase kinases (MKKs) or via TAB1 (MAP3K7IP1)-mediated autophosphorylation. Once activated, it phosphorylates multiple substrates, including transcription factors ATF2, MEF2C, and MAX, as well as cell cycle regulator CDC25B and tumor suppressor p53, highlighting its role in stress-responsive transcription, cell cycle control, and genotoxic stress signaling. Multiple alternatively spliced transcript variants generate distinct MAPK14 isoforms.
