AdipoUpX™ Human ENPP2 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ENPP2 Lentiviral Particle (Overexpression) is professionally engineered for the long-lasting, stable overexpression of the human ENPP2 (Autotaxin) gene. As the enzyme responsible for lysophosphatidic acid production, its stable expression in cell models facilitates investigation into adipocyte proliferation and adipose tissue expansion. The product is ready-to-use and high-titer, with all particles undergoing rigorous internal quality control validation (titer, sterility, and mycoplasma). Our modular design offers high flexibility for the selection of tissue-specific promoters for precise research.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: ENPP2

Full Name: Ectonucleotide pyrophosphatase/phosphodiesterase 2

Gene Symbol: ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA

Gene ID: 5168

RefSeq ID-1: NP_001035181.1

RefSeq ID-2: NM_001040092.3

Summary: ENPP2 functions as both a phosphodiesterase and a phospholipase, specifically catalyzing the production of lysophosphatidic acid (LPA) in extracellular fluids. LPA signals through G-protein coupled receptors to stimulate cell motility and proliferation. Upregulation of this gene is associated with various cancers and chronic inflammation within expanding adipose depots.

AdipoUpX™ Human ENPP2 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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