AdipoUpX™ Human ATF6 MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ATF6 MMLV Particle (Overexpression) is a powerful gene delivery tool designed for the stable overexpression of the human ATF6 gene. As a master regulator of the unfolded protein response (UPR), it is indispensable for researching ER stress-mediated insulin resistance in metabolic tissues. The MMLV vector integrates stably into the host genome, making it an ideal tool for establishing permanent cell lines for longitudinal stress signaling studies. All products undergo comprehensive quality control, including titer, sterility testing, and mycoplasma detection, to ensure experimental safety and high reproducibility.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: ATF6

Full Name: Activating transcription factor 6

Gene Symbol: ACHM7; ATF6A; ATP6alpha

Gene ID: 22926

RefSeq ID-1: NP_031374.2

RefSeq ID-2: NM_007348.4

Summary: Unlike typical transcription factors, ATF6 is synthesized as a permanent resident of the endoplasmic reticulum (ER) membrane. When "unfolded protein stress" occurs, it undergoes proteolysis to release a fragment that travels to the nucleus. This fragment activates the Unfolded Protein Response (UPR), helping the cell survive. In humans, it is a key player in cholesterol balance and has been explored as a factor in diabetes and cystic fibrosis.

AdipoUpX™ Human ATF6 MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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