Rat uncoupling protein 3 (UCP3) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-2085

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: UCP3

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Rat uncoupling protein 3 (UCP3) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Rat uncoupling protein 3 (UCP3) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of UCP3 in rat samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.094 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is based on sandwich enzyme-linked immunosorbent assay technology. An anti-rat UCP3 antibody is pre-immobilized onto a 96-well plate, while a biotin-conjugated anti-rat UCP3 antibody is utilized for detection. Standards, test samples, and the biotinylated detection antibody are added to the wells subsequently and washed with buffer. HRP-streptavidin is then introduced, and unbound components are removed by washing. TMB substrate is used to visualize the HRP enzymatic reaction, producing a blue product that turns yellow after the addition of an acidic stop solution. The resulting color density is proportional to the amount of rat UCP3 captured in the plate. The O.D. absorbance is read at 450 nm, allowing for the calculation of the rat UCP3 concentration.

Background: UCP3 is a key member of the UCP family, functioning as a transporter located in the inner mitochondrial membrane. Its primary role is to create a proton leakage pathway, allowing protons to bypass the ATP synthase and thereby decouple the oxidative phosphorylation process. This decoupling results in energy that would otherwise be used for ATP synthesis being dissipated as heat rather than stored in chemical bonds. In obesity research, UCP3 serves as a key regulator of thermogenesis and systemic energy balance, exhibiting high expression primarily in skeletal muscle. Studies indicate that elevated UCP3 levels may contribute to increased basal metabolic rate, enhanced fatty acid oxidation, and resistance to weight gain, positioning it as a potential therapeutic target for obesity treatment and improved insulin sensitivity.

Synonyms: SLC25A9; Mitochondrial uncoupling protein 3; UCP 3; Solute carrier family 25 member 9; Uncoupling protein 3; mitochondrial

Applications: Rat uncoupling protein 3 (UCP3) ELISA kit (0.156-10 ng/mL) is used to quantify UCP3 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of rat, providing data to support research in a wide range of areas, including metabolism, mitochondrial function, oxidative stress, exercise physiology and energy balance, obesity, and others.

Research Area: Metabolism; Mitochondrial function; Oxidative stress; Exercise physiology and energy balance; Obesity