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Rat uncoupling protein-1 (UCP1) ELISA kit (0.156-10 ng/mL)
Cat. No.:
0126WXX-2047
Assay Type:
Quantitative sandwich ELISA
Target Species:
Rat
Assay Target:
UCP1
Size:
Add to basket
Product Overview
Description:
Rat uncoupling protein-1 (UCP1) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of UCP1 in rat samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.091 ng/mL. The kit is highly sensitive and easy to use.
Assay Principle:
The microtiter plate provided in this kit has been pre-coated with an antibody specific to UCP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for UCP1, followed by the addition of avidin-conjugated HRP. After incubation, a TMB substrate solution is added to each well. Only those wells containing UCP1, biotin-conjugated antibody, and enzyme-conjugated avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UCP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Background:
Uncoupling protein 1 (UCP1) is a mitochondrial transporter that mediates proton leakage across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis and dissipating energy as heat. UCP1 plays a key role in brown adipose tissue thermogenesis and energy expenditure, contributing to the regulation of whole-body metabolism. Impaired UCP1 function can reduce thermogenic capacity and promote energy storage, linking it to obesity and related metabolic disorders.
Synonyms:
SLC25A7; UCP; Thermogenin; Solute carrier family 25 member 7; Uncoupling Protein 1; Mitochondrial; Mitochondrial brown fat uncoupling protein 1
Applications:
Rat uncoupling protein-1 (UCP1) ELISA kit (0.156-10 ng/mL) is used to quantify UCP1 in rat samples, providing data to support research in a wide range of areas, including signal transduction, obesity, and others.
Research Area:
Signal transduction; Obesity
Specification
Sample Type:
Rat samples
Detection Range:
0.156-10 ng/mL
Sensitivity:
0.091 ng/mL
Precision (Intra-assay):
CV≤4.5%
Precision (Inter-assay):
CV≤8.9%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Recovery:
1
Storage:
Store at -20°C.
