Rat phosphoenolpyruvate carboxykinase (PEPCK) ELISA kit-Quantitative sandwich

Cat. No.: 0126WXX-1254

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: PEPCK

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Rat phosphoenolpyruvate carboxykinase (PEPCK) ELISA kit-Quantitative sandwich

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Product Overview

Description: Rat phosphoenolpyruvate carboxykinase (PEPCK) ELISA kit-Quantitative sandwich is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PEPCK in rat samples with a range of 15.625-1000 pg/mL and a minimum detectable dose (sensitivity) of 9.375 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is based on sandwich ELISA technology. An anti-rat PEPCK antibody is pre-coated onto 96-well plates, and a biotin-conjugated anti-rat PEPCK antibody is employed as the detection antibody. Standards, test samples, and the biotinylated detection antibody are added to the wells sequentially, followed by a wash step with wash buffer. HRP-Streptavidin is then introduced, and unbound conjugates are removed through further washing. TMB substrates are used to visualize the HRP enzymatic reaction; the TMB is catalyzed by HRP to produce a blue product that shifts to yellow upon the addition of an acidic stop solution. The intensity of the yellow color is directly proportional to the amount of rat PEPCK captured in the plate. The optical density (O.D.) absorbance is measured at 450 nm in a microplate reader, allowing for the calculation of the rat PEPCK concentration.

Background: PEPCK is a key rate-limiting enzyme in the gluconeogenesis pathway. Its overexpression and increased activity lead to insulin resistance and affect lipid metabolism and obesity.

Synonyms: EC 4.1.1.32; Phosphoenolpyruvate carboxykinase 1; Pepck1; ZDF4

EC NO.: 4.1.1.32

Applications: Rat phosphoenolpyruvate carboxykinase (PEPCK) ELISA kit-Quantitative sandwich is used to quantify PEPCK in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of rat, providing data to support research in a wide range of areas, including metabolism, obesity, and others.

Research Area: Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 15.625-1000 pg/mL

Sensitivity: 9.375 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate; standard) and 2-8°C (other reagents).

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