Rat interleukin 6 (IL-6) ELISA kit (7.8-500 pg/mL)

Cat. No.: 0126WXX-517

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: IL-6

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Rat interleukin 6 (IL-6) ELISA kit (7.8-500 pg/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Rat interleukin 6 (IL-6) ELISA kit (7.8-500 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of IL-6 in rat samples with a range of 7.8-500 pg/mL and a minimum detectable dose (sensitivity) of <3.3 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microtiter plate included in this kit is pre-functionalized with an antibody specific to IL-6. Samples or standards are added to the appropriate wells along with a biotinylated antibody specific for IL-6. Next, avidin-conjugated HRP is added to each microplate well and incubated. After the introduction of the TMB substrate, only those wells containing the IL-6, biotinylated antibody, and enzyme-conjugated avidin will undergo a colorimetric change. The reaction is stopped by adding a sulphuric acid solution, and the color shift is measured at 450 nm ± 10 nm. The final concentration of IL-6 is determined by mapping the O.D. of the samples against the standard curve.

Background: IL-6 is a cytokine with broad biological functions. As a key signaling molecule, it plays a central role in the acute phase response—the body's rapid systemic reaction to inflammation, infection, or injury. Belonging to the IL-6 superfamily, it acts on multiple cell types, including immune cells (B cells, T cells) and hepatocytes. Its primary functions include: immunoregulation and metabolic effects. As a key biological factor in obesity research, IL-6 has demonstrated therapeutic potential for treating obesity.

Synonyms: IL6; MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma growth factor; Hepatocyte stimulating factor; Cytotoxic T-cell differentiation factor

Applications: Rat interleukin 6 (IL-6) ELISA kit (7.8-500 pg/mL) is used to quantify IL-6 in serum, plasma, tissue homogenates, and cell lysates, cell culture supernatants, and other biological fluids of rat, providing data to support research in a wide range of areas, including cytokine, tumor immunity, Infection immunity, cardiovascular biology, obesity, etc.

Research Area: Cytokine; Tumor immunity; Infection immunity; Cardiovascular biology; Obesity