Rat growth hormone (GH) ELISA kit (0.05-30 ng/mL)

Cat. No.: 0126WXX-129

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: GH

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Rat growth hormone (GH) ELISA kit (0.05-30 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Rat growth hormone (GH) ELISA kit (0.05-30 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GH in rat samples with a range of 0.05-30 ng/mL and a minimum detectable dose (sensitivity) of 0.024 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is an ELISA where the plate is pre-coated with a rat GH antibody. GH from the sample binds to the immobilized capture antibody. A biotinylated rat GH antibody is then added, followed by streptavidin-HRP. After washing away unbound conjugates, a substrate solution is added, producing a color change in direct proportion to the amount of rat GH present. The reaction is stopped with an acidic solution and measured spectrophotometrically at 450 nm.

Background: Growth hormone (GH) is a crucial endocrine hormone that plays a key role in growth regulation and metabolic control. It primarily promotes bodily growth by stimulating the liver and other tissues to secrete insulin-like growth factor-1 (IGF-1), while also enhancing amino acid uptake and protein synthesis. Deficiencies in GH can lead to growth hormone deficiency disorders, such as dwarfism. Research indicates that GH induces lipolysis through direct lipolytic effects in adipose tissue and influences obesity to a certain extent.

Synonyms: GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary growth hormone; Growth hormone, normal

Applications: Rat growth hormone (GH) ELISA kit (0.05-30 ng/mL) is used to quantify GH in serum, plasma, cell culture supernates, cell lysates, and tissue homogenates of rat, providing data to support research in a wide range of areas, including developmental biology, obesity, endocrinology, neuro science, metabolism, and others.

Research Area: Developmental biology; Obesity; Endocrinology; Neuro science; Metabolism

Specification

Sample Type: Serum; Plasma; Cell culture supernates; Cell lysates; Tissue homogenates

Detection Range: 0.05-30 ng/mL

Sensitivity: 0.024 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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